Carolina NeuLog Calcium Ion-Selective Manuel d'utilisateur télécharger pdf

NEULOG CALCIUM ION SENSOR GUIDE

NeuLog calcium sensor

NUL-238 Carolina # 369667

The NeuLog calcium ion sensor can be used for any science experiment or activity

which can utilize calcium concentration readings such as in the fields of Environmental

Science, Ecology, Chemistry, Biology, etc.

Just a few of the thousands of possible experimental subjects that can be done with

the NUL-238 sensors are: chemical reactions, water quality, environmental health,

small animal behavior, ecological studies, and many more

The calcium sensor uses the following units of measure:

 Milligrams per liter (mg/L): SI unit of measure for density of a solid dissolved

in a liquid.

 Parts per million (ppm): The total amount of a specific molecule (calcium for

example) per million molecules in a sample.

Calcium sensor usage:

The calcium sensor’s electrode uses a PermaFil (non-refillable), Ion-selective

electrode. The reference chamber is gel filled and sealed off and therefore no further

reference fill solution is required.

Usage guide:

Concentration range

1M to 5x10

-7

0.02 to 40,000 ppm

pH range

2.5 to 11 pH

Temperature range

0 to 40°C

Minimum sample size

3mL in a 50 mL beaker

Reproducibility

±4%

Electrode resistance

1 to 4 megaohms

Required equipment:

 Wash bottle with distilled or deionized water.

 Several clean beakers.

 1 mL, 10 mL, and 100 mL pipettes.

Technical notes:

 All standard solutions should be prepared fresh.

 The standards should be at the same temperature as the sample.

 Use ISA in all solutions.

Note: ISA is added to all solutions to ensure the samples and standards

have the same ionic strength. The ratio is 1:50

Electrode preparation:

1. Remove the protective cap encasing the calcium probe’s electrode.

CAUTION: Do not touch the PVC membrane at the end of the probe

with your fingers

2. Rinse the electrode with deionized water and blot dry. Be sure not to rub as

this will harm the sensor.

3. Soak the electrode in deionized water for 10 minutes, then in a diluted

calcium standard solution (0.0001 M / 1 ppm) until ready for use.

Calibration:

Be sure to calibrate the calcium sensor once daily during periods of regular usage.

1. Prepare the electrode as described in the “Electrode preparation” guide

above.

2. Place 97 mL of deionized water into a 150 mL beaker.

3. Add 2 mL of Ionic Strength Adjustor (ISA) (comes with sensor).

4. Rinse the electrode with deionized water and blot dry and place in the 150

mL beaker.

5. Pipette 1 mL of 1000 ppm calcium standard solution (comes with sensor)

and stir thoroughly.

6. In the NeuLog software, once the sensor has been detected, click on the

calcium sensor icon.

7. After a stable reading has been reached, click on the calibrate button, or

hold the “Start/Stop” button located on the sensors faceplate down for 4

seconds.

8. Rinse electrode once more with deionized water and blot dry.

9. The calcium sensor is now calibrated and ready for use.

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Résumé du contenu

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NEULOG CALCIUM ION SENSOR GUIDE NeuLog calcium sensor NUL-238 Carolina # 369667 The NeuLog calcium ion sensor can be used for any science experim

Page 2

NEULOG CALCIUM ION SENSOR GUIDE Electrode storage: Short term (overnight or weekend): Rinse the electrode thoroughly with deionized water and pla

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NEULOG CALCIUM ION SENSOR GUIDE 5. The WIFI-201 indicator lights will flash; take no action until the LED to the far left turns blue. 6. Take your

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NEULOG CALCIUM ION SENSOR GUIDE Restoring sensor’s factory default settings: Procedure: 1. Connect the USB-200 to a PC, Mac, tablet, or smart dev

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NEULOG CALCIUM ION SENSOR GUIDE Maintenance and storage: Before use, please review the “Calcium sensor usage”, “Electrode preparation”, “E

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Carolina NeuLog Calcium Ion-Selective Manuel d'utilisateur

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