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NEULOG NITRATE SENSOR GUIDE

NeuLog nitrate sensor

NUL-241 Carolina # 369670

The NeuLog nitrate ion sensor can be used for any science experiment or activity

which can utilize nitrate concentration readings such as in the fields of Environmental

Science, Ecology, Chemistry, Biology, etc.

Just a few of the thousands of possible experimental subjects that can be done with

the NUL-241 sensors are: chemical reactions, water quality, environmental health,

small animal behavior, ecological studies, and many more

The nitrate sensor uses the following units of measure:

 Milligrams per liter (mg/L): SI unit of measure for density of a solid dissolved

in a liquid.

 Parts per million (ppm): The total amount of a specific molecule (nitrate for

example) per million molecules in a sample.

Nitrate sensor usage:

The nitrate sensor’s electrode uses a PermaFil (non-refillable), Ion-selective electrode.

The reference chamber is gel filled and sealed and therefore no reference fill solution

is required.

Usage guide:

Concentration range

1M to 7x10

-6

0.1 to 14,000 ppm as N

pH range

2.5 to 11 pH

Temperature range

0 to 50°C

Minimum sample size

3mL in a 50 mL beaker

Reproducibility

±4%

Electrode resistance

1 to 4 megaohms

Required equipment:

 Wash bottle with distilled or deionized water.

 Several clean beakers.

 1 mL, 10 mL, and 100 mL pipettes.

Technical notes:

 All standard solutions should be prepared fresh.

 The standards should be at the same temperature as the sample.

 Use ISA in all solutions.

Note: ISA is added to all solutions to ensure the samples and standards

have the same ionic strength. The ratio is 1:50

Electrode preparation:

1. Remove the protective cap encasing the nitrate probe’s electrode.

CAUTION: Do not touch the PVC membrane at the end of the probe

with your fingers

2. Rinse the electrode with deionized water and blot dry. Be sure not to rub as

this will harm the sensor.

3. Soak the electrode in deionized water for 10 minutes, then in a diluted

nitrate standard solution (either 0.0001 M or 1 ppm) until ready for use.

Calibration:

Be sure to calibrate the nitrate sensor once daily during periods of regular usage.

1. Prepare the electrode as described in the “Electrode preparation” guide

above.

2. Place 97 mL of deionized water into a 150 mL beaker.

3. Add 2 mL of Ionic Strength Adjustor (ISA) (comes with sensor).

4. Rinse the electrode with deionized water and blot dry and place in the 150

mL beaker.

5. Pipette 1 mL of 1000 ppm nitrate standard solution (comes with sensor) and

stir thoroughly.

6. In the NeuLog software, once the sensor has been detected, click on the

nitrate sensor icon.

7. After a stable reading has been reached, click on the calibrate button, or

hold the “Start/Stop” button located on the sensors faceplate down for 4

seconds.

8. Rinse electrode once more with deionized water and blot dry.

9. The nitrate sensor is now calibrated and ready for use.

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NEULOG NITRATE SENSOR GUIDE 1

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Page 1 - NEULOG NITRATE SENSOR GUIDE

NEULOG NITRATE SENSOR GUIDE NeuLog nitrate sensor NUL-241 Carolina # 369670 The NeuLog nitrate ion sensor can be used for any science experiment

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NEULOG NITRATE SENSOR GUIDE Electrode storage: Short term (overnight or weekend): Rinse the electrode thoroughly with deionized water and place

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NEULOG NITRATE SENSOR GUIDE 4. For further WIFI-201 instructions or the WIFI-201 quick start guide please visit www.NeuLog.com/download. 5. The WI

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NEULOG NITRATE SENSOR GUIDE 7. Click the “Load data from sensors” button. 8. Select which experimental data to upload. (5 experimental runs can b

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NEULOG NITRATE SENSOR GUIDE Maintenance and storage: Before use, please review the “Nitrate sensor usage”, “Electrode preparation”, “Elect

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Carolina NeuLog Nitrate Ion-Selective Manuel d'utilisateur

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